masson trichrome stain kit Search Results


mastertech special stain kit  (StatLab Medical Products Inc)
95
StatLab Medical Products Inc mastertech special stain kit
Mastertech Special Stain Kit, supplied by StatLab Medical Products Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mastertech special stain kit/product/StatLab Medical Products Inc
Average 95 stars, based on 1 article reviews
mastertech special stain kit - by Bioz Stars, 2026-03
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trichrome 2000 stain kit  (StatLab Medical Products Inc)
94
StatLab Medical Products Inc trichrome 2000 stain kit
Trichrome 2000 Stain Kit, supplied by StatLab Medical Products Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
trichrome 2000 stain kit - by Bioz Stars, 2026-03
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masson’s trichrome staining g1343  (Beijing Solarbio Science)
90
Beijing Solarbio Science masson’s trichrome staining g1343
Masson’s Trichrome Staining G1343, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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masson’s trichrome staining kit  (ZSGB Biotech)
90
ZSGB Biotech masson’s trichrome staining kit
Masson’s Trichrome Staining Kit, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
masson’s trichrome staining kit - by Bioz Stars, 2026-03
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masson trichrome stain kit  (Polysciences inc)
90
Polysciences inc masson trichrome stain kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson Trichrome Stain Kit, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/masson trichrome stain kit/product/Polysciences inc
Average 90 stars, based on 1 article reviews
masson trichrome stain kit - by Bioz Stars, 2026-03
90/100 stars
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he and masson’s trichrome staining kit  (Nanjing KeyGen Biotech Co Ltd)
90
Nanjing KeyGen Biotech Co Ltd he and masson’s trichrome staining kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
He And Masson’s Trichrome Staining Kit, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/he and masson’s trichrome staining kit/product/Nanjing KeyGen Biotech Co Ltd
Average 90 stars, based on 1 article reviews
he and masson’s trichrome staining kit - by Bioz Stars, 2026-03
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masson’s 2000 trichrome stain kit  (American MasterTech Scientific Inc)
90
American MasterTech Scientific Inc masson’s 2000 trichrome stain kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson’s 2000 Trichrome Stain Kit, supplied by American MasterTech Scientific Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/masson’s 2000 trichrome stain kit/product/American MasterTech Scientific Inc
Average 90 stars, based on 1 article reviews
masson’s 2000 trichrome stain kit - by Bioz Stars, 2026-03
90/100 stars
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masson's trichrome c1006  (Servicebio Inc)
90
Servicebio Inc masson's trichrome c1006
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson's Trichrome C1006, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
masson's trichrome c1006 - by Bioz Stars, 2026-03
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masson's trichrome staining kit  (Keygen Biotech)
90
Keygen Biotech masson's trichrome staining kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson's Trichrome Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/masson's trichrome staining kit/product/Keygen Biotech
Average 90 stars, based on 1 article reviews
masson's trichrome staining kit - by Bioz Stars, 2026-03
90/100 stars
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masson-goldner trichrome staining kit  (Avantor)
90
Avantor masson-goldner trichrome staining kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson Goldner Trichrome Staining Kit, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
masson-goldner trichrome staining kit - by Bioz Stars, 2026-03
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masson’s trichrome staining kit amt.k  (BIOSTAIN READY REAGENTS LIMITED)
90
BIOSTAIN READY REAGENTS LIMITED masson’s trichrome staining kit amt.k
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Masson’s Trichrome Staining Kit Amt.K, supplied by BIOSTAIN READY REAGENTS LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/masson’s trichrome staining kit amt.k/product/BIOSTAIN READY REAGENTS LIMITED
Average 90 stars, based on 1 article reviews
masson’s trichrome staining kit amt.k - by Bioz Stars, 2026-03
90/100 stars
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novaultra masson’s trichrome stain kit  (IHC World)
90
IHC World novaultra masson’s trichrome stain kit
Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, <t>Masson</t> <t>trichrome;</t> TTC, 2,3,5‐triphenyl‐tetrazolium chloride.
Novaultra Masson’s Trichrome Stain Kit, supplied by IHC World, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/novaultra masson’s trichrome stain kit/product/IHC World
Average 90 stars, based on 1 article reviews
novaultra masson’s trichrome stain kit - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, Masson trichrome; TTC, 2,3,5‐triphenyl‐tetrazolium chloride.

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Cardiac Overexpression of S100A6 Attenuates Cardiomyocyte Apoptosis and Reduces Infarct Size After Myocardial Ischemia‐Reperfusion

doi: 10.1161/JAHA.116.004738

Figure Lengend Snippet: Infarct size and apoptosis data post–myocardial ischemia‐reperfusion. A, Representative Evans blue–stained myocardial sections during ligation of the left anterior descending coronary artery, outlining the area at risk in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. B, Representative TTC ‐stained myocardial cryosections 1 day post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The nonviable/infarcted myocardium is white, and viable myocardium is red/pink. C, Representative MTC ‐stained myocardial cryosections 28 days post‐I/R in nontreated controls, empty plasmid–treated controls, and human‐S100A6 plasmid–treated rats. The collagen debris in the central compact necrotic region of the infarcted area is shown in blue, myocardial fibers in pink, and nuclei in purple. D, Risk area by Evans blue as percentage of the LV (mean± SEM , N=6 per group). E, Nonviable myocardium/infarct area by TTC staining as percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=9 per group). F, Infarct area/scar size by MTC staining as a percentage of the LV (mean± SEM , * P <0.05 vs nontreated and empty plasmid–treated controls, N=8‐9 per group). G, IHC staining of infarct region of myocardium 1 day post‐I/R (sarcomeric α‐actinin [red], nuclei [blue], and fluorescein isothiocyanate [ FITC ]‐positive apoptotic nuclei [green]) (magnification ×40). H, Data on apoptotic index in infarct and noninfarct regions in myocardial cryosections 1 day post‐I/R in human‐S100A6 plasmid–treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=7‐9 for each group). I, Caspase 3/7 activity in the infarct and noninfarct regions of myocardium 1 day post‐I/R in S100A6‐treated and empty plasmid–treated rats compared to nontreated controls (mean± SEM , * P <0.05 vs nontreated and empty‐treated controls, N=6 in each group). LV, left ventricle; MTC, Masson trichrome; TTC, 2,3,5‐triphenyl‐tetrazolium chloride.

Article Snippet: We also used Masson Trichrome Stain Kit (Polysciences Inc, Warminster, PA; catalog #25088‐100) to detect collagen fibers (stained blue) and necrotic tissue in the infarcted area of the LV myocardium (stained pink).

Techniques: Staining, Ligation, Plasmid Preparation, Immunohistochemistry, Activity Assay